Identificaciones de pinonbistro

Under an old Quercus robur in a grassy and moss covered area on the side of the road. Culture and dried specimen available at the University of Stirling. ITS from the culture is based off of a Sanger Sequencing reaction of ITS4, since the rxn for the forward primer didn't work for some reason.

VOUCHER # 73204

DATE: 28 NOV 2020

STATE: WA

COUNTY: PIERCE

FORAY ID: South Sound Fungal Diversity Project.

SITE NAME: Forested area just South of the Joint Base Lewis McChord Education Center.

iNat #: 66497034

NEARBY FLORA:

Conifer: Douglas Fir, Hemlock.
Deciduous: Vine Maple, Red Huckleberry.
Evergreen: Sword Fern, Oregon Grape, Rattlesnake Tongue Plantain.

SUBSTRATE: Wood Pieces (likely Conifer), Leaf Litter, Dense Humus Soil, Needle Duff, Moss, Grass.

HABIT: Few (5 within 3-foot area).

LIGHT EXPOSURE: Shade - Partial Shade.

MOISTURE: Wet, .5 inches of cumulative rain in previous 4 days

AMBIENT TEMP: 46 Degrees F

SOIL TEMP: 40 Degrees F

ECOSYSTEM: 2nd-&-3rd Growth Conifer Forest

ELEVATION: 315 Ft/96 Meters

LAY OF THE LAND: Flat, Slightly Irregular

ODOR: Pleasant Mushroom.

TASTE: Unknown, ostensibly Pleasant Mushroom.

REFERENCES:

Mushrooms of the Redwood Coast (Siegel & Schwarz), pg 558;
Mushrooms of the Pacific Northwest (Trudell & Ammirati), pg 280;
Pictorial Key to Pacific NW Mushrooms (Danny Miller)
Mushroom Expert Website (Michael Kuo)

NOTE: There is much discussion in all of these references about the “similarities” of this species w/G. ambigua. The preponderance of the differences are apparently microscopic, but…..there is a distinct difference in Ecosystems…Pine & Sand vs Conifer & Moss substrates.

Hence, in my opinion it would be very-much worthwhile to have these specimens, individually, DNA analyzed. Who knows….there might be 2 different species in this sample.

VOUCHER SPECIMENs SENT TO:

ALDEN DIRKS
U of Michigan
Dept of Ecology & Evolutionary Biology
Ann Arbor, MI

DNA: Confirmed Gyromitra infula.
AAGGATCATTACCATAATGTGCGCTGTCACACGAGTCATCACGCCTGGGC
AGTAATTCCCGCCATATAGTAGGGCAAAAGGCCACCCGGAGTGCCCCCGA
AACAAAACTGTCGGGGGGAGGGCTGCAGTGTCAGCTGGGCTCCGGGGGCG
GGATAGAGTTAGAGTCCCGTCCGCGTCACGCAAGAATCGAAATCGAACTT
GAACCCGGTGCTGGGTTTGCCCGTTAAGGCTGATTGGCCCGTTCACCTTC
ACAAAACCCTCCGTGTACCATTTCCCTGTTGCTTCCCCGCGCCCCCGCGG
CGCGGGGGAGGTCCACCTGAAACACAACAGCACCAAACTGCAGTCAGAAA
AACAAACAAACAATAAGTTAAAACTTTCAACAACGGATCTCTTGGTTCCC
GCATCGATGAAGAACGCAGCGAAATGCGATAAGTAATGTGAATTGCAGAA
TTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCTCTGGTATTCC
GGAGGGCATGCCTGTTCGAGCCTCAATGAAACCGCTCCCCGTGCGGCCGT
TCCTCGAACGTCCCCGGGGGTAGTGGCGGACGCGAACGCCCCCAAAAGCG
TGCGCGCCGCTCAAATCCGCGGTCCGCAGGCCCGAGGGCGCACCCGACGT
AGTGATGAATTTTCTCGTCGACGTGCGACCTCAAGCGCCTGGGACCTTAA
GCGCTCCGGCGCCGCTTACAATCGGTTGAGCTCGGATCAGGTAGG